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Thermo Fisher
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Image Search Results
Journal:
Article Title: Phospholipase D2 Is Localized to the Rims of the Golgi Apparatus in Mammalian Cells
doi: 10.1091/mbc.02-04-0059
Figure Lengend Snippet: PLD2 localization with different organelles in rat NRK cells. Cells were prepared for immunofluorescence microscopy and incubated with rabbit PLD2 antibody PLD2-27 (A, D, G, and J) and costained with monoclonal antibodies to the ER marker BiP (B); the transferrin receptor (E), a marker of early endosomes and the plasma membrane; and lgp120 (H), a late endosome/lysosomal marker. (K) Cells were stained with caveolin-1, a marker for caveoli; the arrow corresponds to caveolin-1 localized to the plasma membrane. Images were merged to determine overlap between PLD2 (red) and the respective marker proteins (green; C, F, I, and L). Areas of maximal overlap are yellow. (L) Inset, enlargement of the region of overlap between PLD2 and caveolin-1 in the perinuclear Golgi apparatus. The arrow indicates areas where PLD2 puncta (red) either overlap completely with caveolin-1 (green) or are directly adjacent. Images are from projected Z-series. Bars, 10 μm.
Article Snippet: Purified monoclonal
Techniques: Immunofluorescence, Microscopy, Incubation, Marker, Staining
Journal: Frontiers in Endocrinology
Article Title: A new LNC89/LNC60-Col11a2 axis revealed by whole-transcriptome analysis may be associated with goiters related to excess iodine nutrition
doi: 10.3389/fendo.2024.1407859
Figure Lengend Snippet: co-expressed mRNA of NONMMUG018089.2.
Article Snippet:
Techniques:
Journal: Frontiers in Endocrinology
Article Title: A new LNC89/LNC60-Col11a2 axis revealed by whole-transcriptome analysis may be associated with goiters related to excess iodine nutrition
doi: 10.3389/fendo.2024.1407859
Figure Lengend Snippet: Col11a2 mRNA and protein expression in goiter mouse model. (A) Col11a2 protein expression levels increased in the 10 weeks treatment group compared with the 10 weeks control group by western blotting. (B) Col11a2 protein expression levels increased in the 20 weeks treatment group compared with the 20 weeks control group by western blotting. (C) Col11a2 expression mRNA levels increased in both treatment groups detected by qRT−PCR. (D) IHC result. (D1) Quantitative analysis of IHC results. Col11a2 expression levels increased in the 10 weeks treatment 1 group compared with 10 weeks control group. Col11a2 expression levels increased in the 20 weeks treatment group compared with the 20 weeks control group. (D2) 10 weeks control group. (D3) 20 weeks control. (D4) 10 weeks treatment. (D5) 20 weeks treatment group. Scale bar = 25 μm. * P < 0.05, ** P < 0.01. *** P < 0.001.
Article Snippet:
Techniques: Expressing, Control, Western Blot, Quantitative RT-PCR
Journal: Frontiers in Endocrinology
Article Title: A new LNC89/LNC60-Col11a2 axis revealed by whole-transcriptome analysis may be associated with goiters related to excess iodine nutrition
doi: 10.3389/fendo.2024.1407859
Figure Lengend Snippet: LNC60-Col11a2 axis in Nthy-ori-1 cells. (A) After treatment with 10 5 and 5×10 6 KIO 3 for 72 h, qPCR showed that LNC60 and Col11a2 mRNA levels were significantly increased compared with those in the control group. (B) With 5×10 6 KIO 3 treated for 72 h, Wb shows Col11a2 protein level was significant increased compared with the Control group. (C) After siLNC60, qPCR showed that LNC60 and Col11a2 were significantly decreased compared with those in the NC group. (D) After siLNC60, Wb showed that the Col11a2 protein level was significantly decreased compared with that in the NC group. (E) After siLNC60, the CCK8 assay showed a significant cell viability inhibition in the 5×10 6 M KIO 3 treatment group at 72 hours. * P <0.05, ** P <0.01, *** P <0.001, t test .
Article Snippet:
Techniques: Control, CCK-8 Assay, Inhibition
Journal: Frontiers in Endocrinology
Article Title: A new LNC89/LNC60-Col11a2 axis revealed by whole-transcriptome analysis may be associated with goiters related to excess iodine nutrition
doi: 10.3389/fendo.2024.1407859
Figure Lengend Snippet: The expression of LNC60 and Col11a2 mRNA in the peripheral blood of Goiter patients and health control populations. *** P< 0.001, **** P< 0.0001, t test.
Article Snippet:
Techniques: Expressing, Control